Monday, March 21, 2022
Reverse Transcriptase demonstrated to incorporate Pfizer mRNA vaccine into cell nuclear DNA in contradiction to Health Experts Claims. 2022-03-21 Jorma Jyrkkanen, BSc, PDP
Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line
Abstract
Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase.
(EXCESS EXPRESSION OF LINE-1 MAY CREATE SERIOUS PROBLEMS FOR CELLS BECAUSE IT MAY LEAD TO OVER-TRANSCRIPTIO; WE JUST DON'T KNOW THE RAMIFICATIONS FOR WHOLE LIVE ORGANISMS-MY COMMENT)Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.
Keywords: COVID-19 mRNA vaccine; BNT162b2; liver; reverse transcription
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Open AccessArticle
Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line
by Markus Aldén
1 [ORCID] , Francisko Olofsson Falla, Daowei Yang, Mohammad Barghouth, Cheng Luan, Magnus Rasmussen and Yang De Marinis
1,* [ORCID]
Department of Clinical Sciences, Lund University, 20502 Malmö, Sweden
Infection Medicine, Department of Clinical Sciences, Lund University, 22362 Lund, Sweden
Author to whom correspondence should be addressed.
Academic Editor: Stephen Malnick
Curr. Issues Mol. Biol. 2022, 44(3), 1115-1126; https://doi.org/10.3390/cimb44030073
Received: 18 January 2022 / Revised: 19 February 2022 / Accepted: 23 February 2022 / Published: 25 February 2022
Compare this to what we are told in Canada to urge our use of vaccines.
Conclusion. We were fed propoganda, not truth. This is so wrong on so many fronts. I actually had shut downs on FB because I tried to post contrary views. There was social media collusion to feed us propoganda and lies. The entry into nuclear DNA means it might also cause nuclear DNA toxicity and lead to cancers and other diseases and there is a possibility that it will cross the placenta into developing fetuses. There is a more sinister possibility as well. The nuclear DNA coding for spike or spike fragments, once incorporated into host genome, may turn on and keep producing spike and the mechanics of that need to be experimentally ruled out.
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